Curriculum vitae

BARRY S. SELINSKY

380 Stonybrook Rd.

King of Prussia, PA 19406

(610) 337-0242

EDUCATION

Lebanon Valley College – Annville, Pennsylvania

Bachelor of Science in Chemistry

Magna cum laude – College and Departmental Honors

Degree conferred – May 1980

State University of New York at Buffalo – Buffalo, New York

Ph.D. – Biochemistry

Conferred – September, 1984

Advisor – Dr. Philip L. Yeagle

PROFESSIONAL EXPERIENCE

August, 2012 – present

Associate Dean for Research and Faculty Development

College of Arts and Sciences, Villanova University

August, 2007 – August, 2012

Professor, Department of Chemistry, Villanova University

Co-Director, Biochemistry Program, Villanova University

August, 2004 – August, 2007

Professor and Chair, Department of Chemistry, Villanova University

August, 2000 – August, 2004

Associate Professor and Chair, Department of Chemistry, Villanova University

September, 1998 – August, 2000

Associate Professor of Chemistry – Villanova University

August, 1998 – July, 1999

Visiting Scholar, Department of Pharmacology, University of Pennsylvania

August, 1988 – August, 1998

Assistant Professor of Chemistry – Villanova University

January, 1989 – September 1990

Applications Consultant – Otsuka Electronics

January, 1988 – July, 1988

Research Assistant Professor – University of North Carolina, Department of Radiology

July, 1985 – July, 1988

Staff Fellow – National Institute of Environmental Health Sciences

Supervisor – Dr. Robert E. London

June, 1984 – July, 1985

Postdoctoral Fellow – Duke University Department of Physiology

Supervisor – Dr. Charles Tanford

HONORS AND SOCIETIES

Who’s Who in American Colleges and Universities – 1980

Graduate Scholar of the State University of New York – 1982-84

Member of the American Chemical Society

Member of the American Society for Biochemistry and Molecular Biology

 

Member of the Editorial Board of Biochimica et Biophysica Acta: Biomembranes 2005-2010.

PUBLICATIONS

Books Edited

  • Membrane Protein Protocols: Expression, Purification and Characterization (B.S. Selinsky, Ed.), Totawa, NJ: Humana Press, 2003.

Submitted Articles

  • Yeagle, P.L., Selinsky, B.S., and Albert, A.D.  Perturbations of phospholipid headgroups by membrane proteins in biological membranes and recombinants.  Biophys. J., 45, 1085-1089, 1984.
  • Selinsky, B.S., and Yeagle, P.L. Two populations of phospholi­pids exist in sarcoplasmic reticulum and in recombined membranes containing the Ca-ATPase. Biochemistry 23, 2281-2288, 1984.
  • Moe, O.A., Pham, S., Selinsky, B.S., and Tang, D. A kinetic study of the effect of Co(II), Cd(II), Cu(II), and Ni(II) ions on the yeast inorganic pyrophosphatase. Biochim. Biophys. Acta 827, 207-214, 1985.
  • Selinsky, B.S., and Yeagle, P.L. Phospholipid exchange between restricted and nonrestricted domains in sarcoplasmic reticulum vesicles. Biochim. Biophys. Acta 813, 33-40, 1985.
  • Frye, J., Albert, A.D., Selinsky, B.S., and Yeagle, P.L.  Cross-polarization P-31 nuclear magnetic resonance of phospholi­pids. Biophys. J. 48, 547-552, 1985.
  • Selinsky, B.S., Thompson, M., and London, R.E. Measurements of in vivo hepatic halothane metabolism in rats using 19F NMR spectroscopy. Biochem. Pharmacol. 36, 413-417, 1987.
  • Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism. I.  Verification and quantitation of methoxydifluoroacetate. Mol.  Pharmacol. 33, 559-566, 1988.
  • Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism.  II. A reevaluation of hepatic metabolic pathways. Mol.  Pharmacol. 33, 567-573, 1988.
  • Selinsky, B.S., Messana, A.D., Scherer, W., and Yeagle, P.L.  Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum. Memb. Biochem. 7, 107-113, 1988.
  • Linderman, R.J., Leaser, J., Roe, R.M., Venkatesh, K., Selinsky, B.S., and London, R.E. 19F NMR spectral evidence that 3-octylthio-1,1,1-trifluoropropan-2-one, a potent inhibitor of insect juvenile hormone esterase, functions as a transition state analog inhibitor. Pest. Biochem. Physiol. 31, 187-194, 1988.
  • Selinsky, B.S., Levy, L.A., Motten, A.G., and London, R.E.  Development of fluorinated, NMR active spin traps for studies of free radical chemistry. J. Mag. Res. 81, 57-67, 1989.
  • Johnson, M., Selinsky, B., Davis, M., Lawrence, T.L., Cleve­land, G., Perry, J.R., Thomas, C., and Kwock, L. In vitro NMR evaluation of human thyroid lesions. Invest. Radiol. 24, 666-671, 1989.
  • Selinsky, B.S., and Yeagle, P.L. Effects of potassium on lipid-protein interactions in light sarcoplasmic reticulum.  Biochemistry 29, 415-421, 1990.
  • Selinsky, B.S., Perlman, M.E., London, R.E., Unkefer, C.J., Mitchell, J., and Blakely, R.L. 13C and 15N Nuclear magnetic resonance evidence of the ionization state of substrates bound to bovine dihydrofolate reductase.  Biochemistry 29, 1290-1296, 1990.
  • Maharaj, G., Selinsky, B.S., Appleman, J.R., Perlman, M., London, R.E., and Blakely, R.L. Dissociation constants for dihydrofolic acid and dihydrobiopterin and implications for mechanistic models for dihydrofolate reductase.  Biochemistry 29, 4554-4560, 1990.
  • Selinsky, B.S., Rusyniak, D.E., Warsheski, J.O., and Joseph, A.P. 19F Nuclear magnetic resonance analysis of trifluoroethanol metabolites in the urine of the Sprague Dawley rat.  Biochem. Pharmacol. 42, 2229-2238, 1991.
  • Jones, S.R., Kinney, W.A., Zhang, X., Jones, L.M., and Selinsky, B.S. The synthesis and characterization of analogs of the antimicrobial compound squalamine: 6b-hydroxy-3-aminosterols synthesized from hyodeoxycholic acid.  Steroids 61, 565-571, 1996.
  • Selinsky, B.S., Zhou, Z., Fotjik, K.G., Jones, S.R., Dollahon, N.R., and Shinnar, A.E. The aminosterol antibiotic squalamine permeabilizes large unilamellar vesicles.  Biochim. Biophys. Acta 1370, 218-234, 1998.
  • Jones, S.R., Selinsky, B.S., Rao, M.N., Zhang, X., Kinney, W.A., and Tham, F.S. Efficient route to 7a-(benzoyloxy)-3-dioxolane cholestan-24(R)-ol, a key intermediate in the synthesis of squalamine.  J. Org. Chem., 63, 3786-3789, 1998.
  • Loll, P.J., Kaplan, J., Selinsky, B.S., and Axelsen, P.H. Vancomycin binding to low affinity ligands: Delineating a minimum set of interactions necessary for high affinity binding.  J. Med. Chem., 42, 4714-4719, 1999.
  • Selinsky, B.S., Smith, R., Frangiosi, A., Vonbaur, B., and Pedersen, L. Squalamine is not a proton ionophore. Biochim. Biophys. Acta, 1464, 135-141, 2000.
  • Selinsky, B.S., Gupta, K., Sharkey, C.T., and Loll, P.J. Structural analysis of NSAID binding by prostaglandin H2 synthase: Time-dependent and time-independent inhibitors elicit identical enzyme conformations. Biochemistry, 40, 5172-5180, 2001.
  • Loll, P.J., Sharkey, C.T., O’Connor, S.J., Dooley, C.M., O’Brien, E., Devocelle, M., Nolan, K.B., Selinsky, B.S., and Fitzgerald, D.J. O-Acetylsalicylhydroxamic acid, a novel acetylating inhibitor of prostaglandin H2 synthase: Structural and functional characterization of enzyme-inhibitor interactions. Mol. Pharmacol., 60, 1407-1413, 2001.
  • Shu, Y., Jones, S.R., Kinney, W.A., and Selinsky, B.S. The synthesis of spermine analogs of the shark aminosterol squalamine. Steroids, 67, 291-304, 2002.
  • Xia, J., Chen, Y., Liberatore, K.M., and Selinsky, B.S. The application of diethylaminosulfur trifluoride in the synthesis of fluorinated sterols and bile acids. Tetrahed. Lett., 44, 9295-9297, 2003.
  • Gupta, K., Selinsky, B.S., Kaub, C.J., and Loll, P.L. The 2.0 A resolution crystal structure of prostaglandin H2 synthase-1: Structural insights into an unusual peroxidase. J. Mol. Biol., 335, 503-518, 2004.
  • Gupta, K., Kaub, C.J., Carey, K.N., Casillas, E.G., Selinsky, B.S., and Loll, P.J. Manipulation of kinetic profiles in 2-aryl propionic acid cyclooxygenase inhibitors. Bioorg. Med. Chem. Lett., 14, 667-671, 2004.
  • Gupta, K., Selinsky, B.S., and Loll, P.J. 2.0 A Crystal structure of prostaglandin H2 synthase-1 reconstituted with a manganese porphyrin cofactor.  Acta Crystallograpica D, 62, 151-156, 2006.
  • Tessema, T-D., Gassler, F., Shu, Y., Jones, S., Selinsky, B.S. Structure-Activity Relationships in Aminosterol Antibiotics: The Effect of Stereochemistry at the 7-OH Group, Bioorg. Med. Chem. Lett., 2013, 23, 3377-3381.
  • Gupta, K., and Selinsky, B.S. Bacterial and algal orthologs of prostaglandin H2 synthase: novel insights into the evolution of an integral membrane protein. Biochim. Biophys. Acta, 1848, 83-94, 2015.

Invited Articles

  • Selinsky, B.S., Gabel, S., Murphy, E., Jeffries, L., and Funk, A. In vivo NMR studies of the hepatic metabolism of xenobiotics. Environ. Health Perspect. 75, 132, 1987.
  • Selinsky, B.S. “Protein-lipid Interactions and Membrane Function”, in The Structure of Biological Membranes (Yeagle, P.L., editor), Boca Raton, FL: CRC Press, 1992, pp. 603-651.
  • Selinsky, B.S., and Burt, C.T.  In vivo 19F NMR.  Biological Magnetic Resonance (Berliner, L.J. and Reuben, J., editors), Volume 11, 1992, pp. 241-276.

Presentations

A total of 50 presentations at national and regional scientific meetings from 1982 – 2015.

RESEARCH GRANTS AWARDED

Supporting Agency: Villanova Faculty Summer Research Award

Project Title: NMR Studies of Hepatic Halothane Metabolism

Date Submitted: October 26, 1988

Amount Awarded: $6000

Description: Fluorine nuclear magnetic resonance will be used to investigate the metabolism of halothane in hepatic microsomal preparations and isolated hepatocytes.

 

Supporting Agency: Villanova Faculty Summer Research Award

Project Title: Tight binding of phospholipid to the calcium-dependent adenosine triphosphatase of sarcoplasmic reticulum.

Date Submitted: November 2, 1991

Amount Awarded: $8000

Description: The identity and functional role of phospholipid tightly bound to the calcium-dependent adenosine triphosphatase will be assessed.

 

Supporting Agency: Magainin Pharmaceuticals

Project Title: Interactions Between Lipids and Antibiotic Peptides: A Lipid Point of View.

Date Submitted: March 5, 1993

Amount Awarded: $42,100

Description: The mechanism of action of the antibiotic peptide magainin 2-amide and analogs will be examined using fluorescence spectroscopy and chemical cross-linking studies.

 

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects.

Effective dates: July 1, 1995 – June 30, 1996

Amount Awarded: $28,242

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

 

Supporting Agency: National Institutes of Health

Project Title: Mechanistic Studies with Aminosterol Antibiotics

Effective Dates: June 1, 1996 – May 31, 2000

Amount Awarded: $102,819

Description: The mechanism of the antibiotic activity of the aminosterol squalamine and analogs will be studied through kinetic analyses and the synthesis of spectroscopic probes.

 

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)

Effective dates: August 1, 1996 – July 31, 1997

Amount Awarded: $30,003

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

 

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)

Effective dates: August 1, 1996 – July 31, 1997

Amount Awarded: $64,443

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

 

Supporting Agency: National Science Foundation

Project Title: Adding Molecular Biology to the Biochemistry Laboratory

Effective dates: March 1, 2001 – February 28, 2002

Amount Awarded: $28,150

Description: An ultracentrifuge and plate reader will be purchased to enhance the instruction of molecular biology in the biochemistry laboratory at Villanova.

 

Supporting Agency: National Science Foundation

Project Title: Acquisition of a Liquid Chromatograph/ Mass Spectrometer

Effective dates: September 1, 2006 – August 31, 2008

Amount Awarded: $329,236

Description: An LC/MS will be purchased to support research and instruction in the Chemistry Department at Villanova University.

 

OUTSIDE INTERESTS

 Golf, distance running, gardening

 

Membrane proteins and membrane-active agents