Curriculum vitae

BARRY S. SELINSKY

380 Stonybrook Rd.

King of Prussia, PA 19406

(610) 337-0242

EDUCATION

Lebanon Valley College – Annville, Pennsylvania

Bachelor of Science in Chemistry

Magna cum laude – College and Departmental Honors

Degree conferred – May 1980

State University of New York at Buffalo – Buffalo, New York

Ph.D. – Biochemistry

Conferred – September, 1984

Advisor – Dr. Philip L. Yeagle

PROFESSIONAL EXPERIENCE

August, 2012 – present

Associate Dean for Research and Faculty Development

College of Arts and Sciences, Villanova University

August, 2007 – August, 2012

Professor, Department of Chemistry, Villanova University

Co-Director, Biochemistry Program, Villanova University

August, 2004 – August, 2007

Professor and Chair, Department of Chemistry, Villanova University

August, 2000 – August, 2004

Associate Professor and Chair, Department of Chemistry, Villanova University

September, 1998 – August, 2000

Associate Professor of Chemistry – Villanova University

August, 1998 – July, 1999

Visiting Scholar, Department of Pharmacology, University of Pennsylvania

August, 1988 – August, 1998

Assistant Professor of Chemistry – Villanova University

January, 1989 – September 1990

Applications Consultant – Otsuka Electronics

January, 1988 – July, 1988

Research Assistant Professor – University of North Carolina, Department of Radiology

July, 1985 – July, 1988

Staff Fellow – National Institute of Environmental Health Sciences

Supervisor – Dr. Robert E. London

June, 1984 – July, 1985

Postdoctoral Fellow – Duke University Department of Physiology

Supervisor – Dr. Charles Tanford

HONORS AND SOCIETIES

Who’s Who in American Colleges and Universities – 1980

Graduate Scholar of the State University of New York – 1982-84

Member of the American Chemical Society

Member of the American Society for Biochemistry and Molecular Biology

Member of the Editorial Board of Biochimica et Biophysica Acta: Biomembranes 2005-2010.

PUBLICATIONS

Books Edited

  • Membrane Protein Protocols: Expression, Purification and Characterization (B.S. Selinsky, Ed.), Totawa, NJ: Humana Press, 2003.

Submitted Articles

  • Yeagle, P.L., Selinsky, B.S., and Albert, A.D.  Perturbations of phospholipid headgroups by membrane proteins in biological membranes and recombinants.  Biophys. J., 45, 1085-1089, 1984.
  • Selinsky, B.S., and Yeagle, P.L. Two populations of phospholi­pids exist in sarcoplasmic reticulum and in recombined membranes containing the Ca-ATPase. Biochemistry 23, 2281-2288, 1984.
  • Moe, O.A., Pham, S., Selinsky, B.S., and Tang, D. A kinetic study of the effect of Co(II), Cd(II), Cu(II), and Ni(II) ions on the yeast inorganic pyrophosphatase. Biochim. Biophys. Acta 827, 207-214, 1985.
  • Selinsky, B.S., and Yeagle, P.L. Phospholipid exchange between restricted and nonrestricted domains in sarcoplasmic reticulum vesicles. Biochim. Biophys. Acta 813, 33-40, 1985.
  • Frye, J., Albert, A.D., Selinsky, B.S., and Yeagle, P.L.  Cross-polarization P-31 nuclear magnetic resonance of phospholi­pids. Biophys. J. 48, 547-552, 1985.
  • Selinsky, B.S., Thompson, M., and London, R.E. Measurements of in vivo hepatic halothane metabolism in rats using 19F NMR spectroscopy. Biochem. Pharmacol. 36, 413-417, 1987.
  • Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism. I.  Verification and quantitation of methoxydifluoroacetate. Mol.  Pharmacol. 33, 559-566, 1988.
  • Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism.  II. A reevaluation of hepatic metabolic pathways. Mol.  Pharmacol. 33, 567-573, 1988.
  • Selinsky, B.S., Messana, A.D., Scherer, W., and Yeagle, P.L.  Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum. Memb. Biochem. 7, 107-113, 1988.
  • Linderman, R.J., Leaser, J., Roe, R.M., Venkatesh, K., Selinsky, B.S., and London, R.E. 19F NMR spectral evidence that 3-octylthio-1,1,1-trifluoropropan-2-one, a potent inhibitor of insect juvenile hormone esterase, functions as a transition state analog inhibitor. Pest. Biochem. Physiol. 31, 187-194, 1988.
  • Selinsky, B.S., Levy, L.A., Motten, A.G., and London, R.E.  Development of fluorinated, NMR active spin traps for studies of free radical chemistry. J. Mag. Res. 81, 57-67, 1989.
  • Johnson, M., Selinsky, B., Davis, M., Lawrence, T.L., Cleve­land, G., Perry, J.R., Thomas, C., and Kwock, L. In vitro NMR evaluation of human thyroid lesions. Invest. Radiol. 24, 666-671, 1989.
  • Selinsky, B.S., and Yeagle, P.L. Effects of potassium on lipid-protein interactions in light sarcoplasmic reticulum.  Biochemistry 29, 415-421, 1990.
  • Selinsky, B.S., Perlman, M.E., London, R.E., Unkefer, C.J., Mitchell, J., and Blakely, R.L. 13C and 15N Nuclear magnetic resonance evidence of the ionization state of substrates bound to bovine dihydrofolate reductase.  Biochemistry 29, 1290-1296, 1990.
  • Maharaj, G., Selinsky, B.S., Appleman, J.R., Perlman, M., London, R.E., and Blakely, R.L. Dissociation constants for dihydrofolic acid and dihydrobiopterin and implications for mechanistic models for dihydrofolate reductase.  Biochemistry 29, 4554-4560, 1990.
  • Selinsky, B.S., Rusyniak, D.E., Warsheski, J.O., and Joseph, A.P. 19F Nuclear magnetic resonance analysis of trifluoroethanol metabolites in the urine of the Sprague Dawley rat.  Biochem. Pharmacol. 42, 2229-2238, 1991.
  • Jones, S.R., Kinney, W.A., Zhang, X., Jones, L.M., and Selinsky, B.S. The synthesis and characterization of analogs of the antimicrobial compound squalamine: 6b-hydroxy-3-aminosterols synthesized from hyodeoxycholic acid.  Steroids 61, 565-571, 1996.
  • Selinsky, B.S., Zhou, Z., Fotjik, K.G., Jones, S.R., Dollahon, N.R., and Shinnar, A.E. The aminosterol antibiotic squalamine permeabilizes large unilamellar vesicles.  Biochim. Biophys. Acta 1370, 218-234, 1998.
  • Jones, S.R., Selinsky, B.S., Rao, M.N., Zhang, X., Kinney, W.A., and Tham, F.S. Efficient route to 7a-(benzoyloxy)-3-dioxolane cholestan-24(R)-ol, a key intermediate in the synthesis of squalamine.  J. Org. Chem., 63, 3786-3789, 1998.
  • Loll, P.J., Kaplan, J., Selinsky, B.S., and Axelsen, P.H. Vancomycin binding to low affinity ligands: Delineating a minimum set of interactions necessary for high affinity binding.  J. Med. Chem., 42, 4714-4719, 1999.
  • Selinsky, B.S., Smith, R., Frangiosi, A., Vonbaur, B., and Pedersen, L. Squalamine is not a proton ionophore. Biochim. Biophys. Acta, 1464, 135-141, 2000.
  • Selinsky, B.S., Gupta, K., Sharkey, C.T., and Loll, P.J. Structural analysis of NSAID binding by prostaglandin H2 synthase: Time-dependent and time-independent inhibitors elicit identical enzyme conformations. Biochemistry, 40, 5172-5180, 2001.
  • Loll, P.J., Sharkey, C.T., O’Connor, S.J., Dooley, C.M., O’Brien, E., Devocelle, M., Nolan, K.B., Selinsky, B.S., and Fitzgerald, D.J. O-Acetylsalicylhydroxamic acid, a novel acetylating inhibitor of prostaglandin H2 synthase: Structural and functional characterization of enzyme-inhibitor interactions. Mol. Pharmacol., 60, 1407-1413, 2001.
  • Shu, Y., Jones, S.R., Kinney, W.A., and Selinsky, B.S. The synthesis of spermine analogs of the shark aminosterol squalamine. Steroids, 67, 291-304, 2002.
  • Xia, J., Chen, Y., Liberatore, K.M., and Selinsky, B.S. The application of diethylaminosulfur trifluoride in the synthesis of fluorinated sterols and bile acids. Tetrahed. Lett., 44, 9295-9297, 2003.
  • Gupta, K., Selinsky, B.S., Kaub, C.J., and Loll, P.L. The 2.0 A resolution crystal structure of prostaglandin H2 synthase-1: Structural insights into an unusual peroxidase. J. Mol. Biol., 335, 503-518, 2004.
  • Gupta, K., Kaub, C.J., Carey, K.N., Casillas, E.G., Selinsky, B.S., and Loll, P.J. Manipulation of kinetic profiles in 2-aryl propionic acid cyclooxygenase inhibitors. Bioorg. Med. Chem. Lett., 14, 667-671, 2004.
  • Gupta, K., Selinsky, B.S., and Loll, P.J. 2.0 A Crystal structure of prostaglandin H2 synthase-1 reconstituted with a manganese porphyrin cofactor.  Acta Crystallograpica D, 62, 151-156, 2006.
  • Tessema, T-D., Gassler, F., Shu, Y., Jones, S., Selinsky, B.S. Structure-Activity Relationships in Aminosterol Antibiotics: The Effect of Stereochemistry at the 7-OH Group, Bioorg. Med. Chem. Lett., 2013, 23, 3377-3381.
  • Gupta, K., and Selinsky, B.S. Bacterial and algal orthologs of prostaglandin H2 synthase: novel insights into the evolution of an integral membrane protein. Biochim. Biophys. Acta, 1848, 83-94, 2015.

Invited Articles

  • Selinsky, B.S., Gabel, S., Murphy, E., Jeffries, L., and Funk, A. In vivo NMR studies of the hepatic metabolism of xenobiotics. Environ. Health Perspect. 75, 132, 1987.
  • Selinsky, B.S. “Protein-lipid Interactions and Membrane Function”, in The Structure of Biological Membranes (Yeagle, P.L., editor), Boca Raton, FL: CRC Press, 1992, pp. 603-651.
  • Selinsky, B.S., and Burt, C.T.  In vivo 19F NMR.  Biological Magnetic Resonance (Berliner, L.J. and Reuben, J., editors), Volume 11, 1992, pp. 241-276.

Presentations

A total of 56 presentations at national and regional scientific meetings from 1982 – 2019.

RESEARCH GRANTS AWARDED

Supporting Agency: Villanova Faculty Summer Research Award

Project Title: NMR Studies of Hepatic Halothane Metabolism

Date Submitted: October 26, 1988

Amount Awarded: $6000

Description: Fluorine nuclear magnetic resonance will be used to investigate the metabolism of halothane in hepatic microsomal preparations and isolated hepatocytes.

Supporting Agency: Villanova Faculty Summer Research Award

Project Title: Tight binding of phospholipid to the calcium-dependent adenosine triphosphatase of sarcoplasmic reticulum.

Date Submitted: November 2, 1991

Amount Awarded: $8000

Description: The identity and functional role of phospholipid tightly bound to the calcium-dependent adenosine triphosphatase will be assessed.

Supporting Agency: Magainin Pharmaceuticals

Project Title: Interactions Between Lipids and Antibiotic Peptides: A Lipid Point of View.

Date Submitted: March 5, 1993

Amount Awarded: $42,100

Description: The mechanism of action of the antibiotic peptide magainin 2-amide and analogs will be examined using fluorescence spectroscopy and chemical cross-linking studies.

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects.

Effective dates: July 1, 1995 – June 30, 1996

Amount Awarded: $28,242

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

Supporting Agency: National Institutes of Health

Project Title: Mechanistic Studies with Aminosterol Antibiotics

Effective Dates: June 1, 1996 – May 31, 2000

Amount Awarded: $102,819

Description: The mechanism of the antibiotic activity of the aminosterol squalamine and analogs will be studied through kinetic analyses and the synthesis of spectroscopic probes.

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)

Effective dates: August 1, 1996 – July 31, 1997

Amount Awarded: $30,003

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

Supporting Agency: Magainin Pharmaceuticals

Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)

Effective dates: August 1, 1996 – July 31, 1997

Amount Awarded: $64,443

Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.

Supporting Agency: National Science Foundation

Project Title: Adding Molecular Biology to the Biochemistry Laboratory

Effective dates: March 1, 2001 – February 28, 2002

Amount Awarded: $28,150

Description: An ultracentrifuge and plate reader will be purchased to enhance the instruction of molecular biology in the biochemistry laboratory at Villanova.

Supporting Agency: National Science Foundation

Project Title: Acquisition of a Liquid Chromatograph/ Mass Spectrometer

Effective dates: September 1, 2006 – August 31, 2008

Amount Awarded: $329,236

Description: An LC/MS will be purchased to support research and instruction in the Chemistry Department at Villanova University.

Supporting Agency: Arnold and Mabel Beckman Foundation Beckman Scholars Program

Project Title: Beckman Scholars Program at Villanova University

Effective dates: June 1, 2017 – August 31, 2020

Amount Awarded: $130,000

Description: Funds are provided to support undergraduate research students and their mentors as the students pursue directed research projects at Villanova University.

OUTSIDE INTERESTS

 Golf, distance running, gardening

 

Membrane proteins and membrane-active agents