BARRY S. SELINSKY
380 Stonybrook Rd.
King of Prussia, PA 19406
(610) 337-0242
EDUCATION
Lebanon Valley College – Annville, Pennsylvania
Bachelor of Science in Chemistry
Magna cum laude – College and Departmental Honors
Degree conferred – May 1980
State University of New York at Buffalo – Buffalo, New York
Ph.D. – Biochemistry
Conferred – September, 1984
Advisor – Dr. Philip L. Yeagle
PROFESSIONAL EXPERIENCE
August, 2012 – present
Associate Dean for Research and Faculty Development
College of Arts and Sciences, Villanova University
August, 2007 – August, 2012
Professor, Department of Chemistry, Villanova University
Co-Director, Biochemistry Program, Villanova University
August, 2004 – August, 2007
Professor and Chair, Department of Chemistry, Villanova University
August, 2000 – August, 2004
Associate Professor and Chair, Department of Chemistry, Villanova University
September, 1998 – August, 2000
Associate Professor of Chemistry – Villanova University
August, 1998 – July, 1999
Visiting Scholar, Department of Pharmacology, University of Pennsylvania
August, 1988 – August, 1998
Assistant Professor of Chemistry – Villanova University
January, 1989 – September 1990
Applications Consultant – Otsuka Electronics
January, 1988 – July, 1988
Research Assistant Professor – University of North Carolina, Department of Radiology
July, 1985 – July, 1988
Staff Fellow – National Institute of Environmental Health Sciences
Supervisor – Dr. Robert E. London
June, 1984 – July, 1985
Postdoctoral Fellow – Duke University Department of Physiology
Supervisor – Dr. Charles Tanford
HONORS AND SOCIETIES
Who’s Who in American Colleges and Universities – 1980
Graduate Scholar of the State University of New York – 1982-84
Member of the American Chemical Society
Member of the American Society for Biochemistry and Molecular Biology
Member of the Editorial Board of Biochimica et Biophysica Acta: Biomembranes 2005-2010.
PUBLICATIONS
Books Edited
- Membrane Protein Protocols: Expression, Purification and Characterization (B.S. Selinsky, Ed.), Totawa, NJ: Humana Press, 2003.
Submitted Articles
- Yeagle, P.L., Selinsky, B.S., and Albert, A.D. Perturbations of phospholipid headgroups by membrane proteins in biological membranes and recombinants. Biophys. J., 45, 1085-1089, 1984.
- Selinsky, B.S., and Yeagle, P.L. Two populations of phospholipids exist in sarcoplasmic reticulum and in recombined membranes containing the Ca-ATPase. Biochemistry 23, 2281-2288, 1984.
- Moe, O.A., Pham, S., Selinsky, B.S., and Tang, D. A kinetic study of the effect of Co(II), Cd(II), Cu(II), and Ni(II) ions on the yeast inorganic pyrophosphatase. Biochim. Biophys. Acta 827, 207-214, 1985.
- Selinsky, B.S., and Yeagle, P.L. Phospholipid exchange between restricted and nonrestricted domains in sarcoplasmic reticulum vesicles. Biochim. Biophys. Acta 813, 33-40, 1985.
- Frye, J., Albert, A.D., Selinsky, B.S., and Yeagle, P.L. Cross-polarization P-31 nuclear magnetic resonance of phospholipids. Biophys. J. 48, 547-552, 1985.
- Selinsky, B.S., Thompson, M., and London, R.E. Measurements of in vivo hepatic halothane metabolism in rats using 19F NMR spectroscopy. Biochem. Pharmacol. 36, 413-417, 1987.
- Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism. I. Verification and quantitation of methoxydifluoroacetate. Mol. Pharmacol. 33, 559-566, 1988.
- Selinsky, B.S., Perlman, M.E., and London, R.E. In vivo nuclear magnetic resonance studies of hepatic methoxyflurane metabolism. II. A reevaluation of hepatic metabolic pathways. Mol. Pharmacol. 33, 567-573, 1988.
- Selinsky, B.S., Messana, A.D., Scherer, W., and Yeagle, P.L. Effects of TRIS and HEPES on function of rabbit muscle light sarcoplasmic reticulum. Memb. Biochem. 7, 107-113, 1988.
- Linderman, R.J., Leaser, J., Roe, R.M., Venkatesh, K., Selinsky, B.S., and London, R.E. 19F NMR spectral evidence that 3-octylthio-1,1,1-trifluoropropan-2-one, a potent inhibitor of insect juvenile hormone esterase, functions as a transition state analog inhibitor. Pest. Biochem. Physiol. 31, 187-194, 1988.
- Selinsky, B.S., Levy, L.A., Motten, A.G., and London, R.E. Development of fluorinated, NMR active spin traps for studies of free radical chemistry. J. Mag. Res. 81, 57-67, 1989.
- Johnson, M., Selinsky, B., Davis, M., Lawrence, T.L., Cleveland, G., Perry, J.R., Thomas, C., and Kwock, L. In vitro NMR evaluation of human thyroid lesions. Invest. Radiol. 24, 666-671, 1989.
- Selinsky, B.S., and Yeagle, P.L. Effects of potassium on lipid-protein interactions in light sarcoplasmic reticulum. Biochemistry 29, 415-421, 1990.
- Selinsky, B.S., Perlman, M.E., London, R.E., Unkefer, C.J., Mitchell, J., and Blakely, R.L. 13C and 15N Nuclear magnetic resonance evidence of the ionization state of substrates bound to bovine dihydrofolate reductase. Biochemistry 29, 1290-1296, 1990.
- Maharaj, G., Selinsky, B.S., Appleman, J.R., Perlman, M., London, R.E., and Blakely, R.L. Dissociation constants for dihydrofolic acid and dihydrobiopterin and implications for mechanistic models for dihydrofolate reductase. Biochemistry 29, 4554-4560, 1990.
- Selinsky, B.S., Rusyniak, D.E., Warsheski, J.O., and Joseph, A.P. 19F Nuclear magnetic resonance analysis of trifluoroethanol metabolites in the urine of the Sprague Dawley rat. Biochem. Pharmacol. 42, 2229-2238, 1991.
- Jones, S.R., Kinney, W.A., Zhang, X., Jones, L.M., and Selinsky, B.S. The synthesis and characterization of analogs of the antimicrobial compound squalamine: 6b-hydroxy-3-aminosterols synthesized from hyodeoxycholic acid. Steroids 61, 565-571, 1996.
- Selinsky, B.S., Zhou, Z., Fotjik, K.G., Jones, S.R., Dollahon, N.R., and Shinnar, A.E. The aminosterol antibiotic squalamine permeabilizes large unilamellar vesicles. Biochim. Biophys. Acta 1370, 218-234, 1998.
- Jones, S.R., Selinsky, B.S., Rao, M.N., Zhang, X., Kinney, W.A., and Tham, F.S. Efficient route to 7a-(benzoyloxy)-3-dioxolane cholestan-24(R)-ol, a key intermediate in the synthesis of squalamine. J. Org. Chem., 63, 3786-3789, 1998.
- Loll, P.J., Kaplan, J., Selinsky, B.S., and Axelsen, P.H. Vancomycin binding to low affinity ligands: Delineating a minimum set of interactions necessary for high affinity binding. J. Med. Chem., 42, 4714-4719, 1999.
- Selinsky, B.S., Smith, R., Frangiosi, A., Vonbaur, B., and Pedersen, L. Squalamine is not a proton ionophore. Biochim. Biophys. Acta, 1464, 135-141, 2000.
- Selinsky, B.S., Gupta, K., Sharkey, C.T., and Loll, P.J. Structural analysis of NSAID binding by prostaglandin H2 synthase: Time-dependent and time-independent inhibitors elicit identical enzyme conformations. Biochemistry, 40, 5172-5180, 2001.
- Loll, P.J., Sharkey, C.T., O’Connor, S.J., Dooley, C.M., O’Brien, E., Devocelle, M., Nolan, K.B., Selinsky, B.S., and Fitzgerald, D.J. O-Acetylsalicylhydroxamic acid, a novel acetylating inhibitor of prostaglandin H2 synthase: Structural and functional characterization of enzyme-inhibitor interactions. Mol. Pharmacol., 60, 1407-1413, 2001.
- Shu, Y., Jones, S.R., Kinney, W.A., and Selinsky, B.S. The synthesis of spermine analogs of the shark aminosterol squalamine. Steroids, 67, 291-304, 2002.
- Xia, J., Chen, Y., Liberatore, K.M., and Selinsky, B.S. The application of diethylaminosulfur trifluoride in the synthesis of fluorinated sterols and bile acids. Tetrahed. Lett., 44, 9295-9297, 2003.
- Gupta, K., Selinsky, B.S., Kaub, C.J., and Loll, P.L. The 2.0 A resolution crystal structure of prostaglandin H2 synthase-1: Structural insights into an unusual peroxidase. J. Mol. Biol., 335, 503-518, 2004.
- Gupta, K., Kaub, C.J., Carey, K.N., Casillas, E.G., Selinsky, B.S., and Loll, P.J. Manipulation of kinetic profiles in 2-aryl propionic acid cyclooxygenase inhibitors. Bioorg. Med. Chem. Lett., 14, 667-671, 2004.
- Gupta, K., Selinsky, B.S., and Loll, P.J. 2.0 A Crystal structure of prostaglandin H2 synthase-1 reconstituted with a manganese porphyrin cofactor. Acta Crystallograpica D, 62, 151-156, 2006.
- Tessema, T-D., Gassler, F., Shu, Y., Jones, S., Selinsky, B.S. Structure-Activity Relationships in Aminosterol Antibiotics: The Effect of Stereochemistry at the 7-OH Group, Bioorg. Med. Chem. Lett., 2013, 23, 3377-3381.
- Gupta, K., and Selinsky, B.S. Bacterial and algal orthologs of prostaglandin H2 synthase: novel insights into the evolution of an integral membrane protein. Biochim. Biophys. Acta, 1848, 83-94, 2015.
Invited Articles
- Selinsky, B.S., Gabel, S., Murphy, E., Jeffries, L., and Funk, A. In vivo NMR studies of the hepatic metabolism of xenobiotics. Environ. Health Perspect. 75, 132, 1987.
- Selinsky, B.S. “Protein-lipid Interactions and Membrane Function”, in The Structure of Biological Membranes (Yeagle, P.L., editor), Boca Raton, FL: CRC Press, 1992, pp. 603-651.
- Selinsky, B.S., and Burt, C.T. In vivo 19F NMR. Biological Magnetic Resonance (Berliner, L.J. and Reuben, J., editors), Volume 11, 1992, pp. 241-276.
Presentations
A total of 56 presentations at national and regional scientific meetings from 1982 – 2019.
RESEARCH GRANTS AWARDED
Supporting Agency: Villanova Faculty Summer Research Award
Project Title: NMR Studies of Hepatic Halothane Metabolism
Date Submitted: October 26, 1988
Amount Awarded: $6000
Description: Fluorine nuclear magnetic resonance will be used to investigate the metabolism of halothane in hepatic microsomal preparations and isolated hepatocytes.
Supporting Agency: Villanova Faculty Summer Research Award
Project Title: Tight binding of phospholipid to the calcium-dependent adenosine triphosphatase of sarcoplasmic reticulum.
Date Submitted: November 2, 1991
Amount Awarded: $8000
Description: The identity and functional role of phospholipid tightly bound to the calcium-dependent adenosine triphosphatase will be assessed.
Supporting Agency: Magainin Pharmaceuticals
Project Title: Interactions Between Lipids and Antibiotic Peptides: A Lipid Point of View.
Date Submitted: March 5, 1993
Amount Awarded: $42,100
Description: The mechanism of action of the antibiotic peptide magainin 2-amide and analogs will be examined using fluorescence spectroscopy and chemical cross-linking studies.
Supporting Agency: Magainin Pharmaceuticals
Project Title: MPI Compounds and MPI Information to Perform MPI Projects.
Effective dates: July 1, 1995 – June 30, 1996
Amount Awarded: $28,242
Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.
Supporting Agency: National Institutes of Health
Project Title: Mechanistic Studies with Aminosterol Antibiotics
Effective Dates: June 1, 1996 – May 31, 2000
Amount Awarded: $102,819
Description: The mechanism of the antibiotic activity of the aminosterol squalamine and analogs will be studied through kinetic analyses and the synthesis of spectroscopic probes.
Supporting Agency: Magainin Pharmaceuticals
Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)
Effective dates: August 1, 1996 – July 31, 1997
Amount Awarded: $30,003
Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.
Supporting Agency: Magainin Pharmaceuticals
Project Title: MPI Compounds and MPI Information to Perform MPI Projects (Continuation)
Effective dates: August 1, 1996 – July 31, 1997
Amount Awarded: $64,443
Description: Analogs of the aminosterol antibiotic squalamine will be synthesized for testing by Magainin Pharmaceuticals.
Supporting Agency: National Science Foundation
Project Title: Adding Molecular Biology to the Biochemistry Laboratory
Effective dates: March 1, 2001 – February 28, 2002
Amount Awarded: $28,150
Description: An ultracentrifuge and plate reader will be purchased to enhance the instruction of molecular biology in the biochemistry laboratory at Villanova.
Supporting Agency: National Science Foundation
Project Title: Acquisition of a Liquid Chromatograph/ Mass Spectrometer
Effective dates: September 1, 2006 – August 31, 2008
Amount Awarded: $329,236
Description: An LC/MS will be purchased to support research and instruction in the Chemistry Department at Villanova University.
Supporting Agency: Arnold and Mabel Beckman Foundation Beckman Scholars Program
Project Title: Beckman Scholars Program at Villanova University
Effective dates: June 1, 2017 – August 31, 2020
Amount Awarded: $130,000
Description: Funds are provided to support undergraduate research students and their mentors as the students pursue directed research projects at Villanova University.
OUTSIDE INTERESTS
Golf, distance running, gardening